Roundup (glyphosate) works best on perennials in the fall just as they are turning colors / browning up for the winter - or just after they begin to turn.
This is because, at that time the plant will start to translocate all of the food and nutrients in the leaves and stalk to the roots for over winter storage. Glyphosate hitches a ride to the roots and kills the whole plant (or close to it).
My dad sprayed a big patch of poison ivy with Round Up in June one year, all the leaves fell of then grew right back. I spray poison ivy in September and I kill just about every little darn plant.
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